Morphology of Stems – PST05210 Basic Pharmacognosy

NTA Level 5 • Semester 2 • PST05210

Morphology of Stems

Basic Pharmacognosy • Source Session/Topic 6
Full source-text version: all educational wording from the extracted learning source is retained; only presenter/tutor metadata and web-layout noise are removed, while formatting is improved for readability.

Session 6: Morphology of Stems

Total Session Time: 120 minutes

Prerequisites

• None

Learning Tasks

By the end of this session students are expected to be able to:

• Introduction of the Stem
• External Structure of the Stem
• Differentiate Dicot from Monocot Stem
• Functions of the Stem

Resources Needed:

• Flip charts, marker pens, and masking tape
• Black/white board and chalk/whiteboard markers
• LCD projector and laptop

SESSION OVERVIEW

|Step |Time |Activity/ |Content |

| | |Method | |

|1 |05 minutes |Presentation |Introduction, Learning Tasks |

|2 |20 minutes |Presentation |Introduction to Stems and External|

| | |Brainstorming |Features |

|3 |35 minutes |Presentation |Internal Structures of Dicot Stems|

|4 |30 minutes |Presentation |Internal Structures of Monocot |

| | | |Stems |

|5 |20 minutes |Presentation |Differences between Monocot and |

| | |Small group |Dicot Stems |

| | |discussion | |

|6 |05 minutes |Presentation |Key Points |

|7 |05 minutes |Presentation |Evaluation |

SESSION CONTENTS

STEP 1: Presentation of Session Title and Learning Tasks (5 minutes)

READ or ASK students to read the learning objectives and clarify

ASK students if they have any questions before continuing.

STEP 2: Introduction to Stems and External Features (20 minutes)

• Stem is the axis of the plant other than the root
• In most plants stems are located above the soil surface but some plants

have underground stems

• Functions of the stem

o Stems support the leaves and other structures e.g. flowers,

fruits

o Stems are used for vegetative reproductive i.e. cuttings

o Stems position leaves to receive maximum sunlight

o Stems determine size and shape of the plant

o Facilitates movement of water, minerals, and manufactured

food in the plant

o Some stems carry out photosynthesis

o Stores foods

|Activity: Brainstorming (5 minutes) |

| |

|Ask students to brainstorm on the following question: |

| |

|What are the external structures of the stem? |

| |

|ALLOW few students to respond? |

| |

|WRITE their responses on the flip chart/ board |

| |

|CLARIFY and SUMMARISE by using the content below |

• External structures of the stem

o Internodes: distances separating one node from

another

o Nodes: Points at which leaves are attached

o Lenticels: Breathing pores

o Buds: Growth of shoot

o Terminal or apical but, at tip of plan

o Axillary bud in nodes on stem

o Bud scale scars: Indicate where terminal bud has been located previous

year

o Leaf scars:

o Show where leaf was attached

• Internal structures of the stem

o Usually consist of three tissues, Dermal tissue, Ground

tissue and Vascular tissue

o The dermal tissue

▪ Covers outer surface of stem and usually functions to waterproof,

protect & control gas exchange

o The Ground tissue

▪ Mainly of parenchyma cells and fills in around the vascular tissue,

sometimes functions in photosynthesis

o The Vascular tissue

▪ Provides long distance transport and structural support
▪ Arrangement varies widely among plant species

STEP 3: Internal Structures of the Dicot Stem (35 minutes)

• Internal structure of dicotyledonous stem consists of Epidermis, Cortex

and Stele.

o Epidermis

▪ It consists of a single layer of parenchymatous rectangular cells
▪ Epidermal cells are compactly arranged without intercellular spaces
▪ Epidermis is covered on the outside by cuticle made up of waxy

substance

▪ The cuticle checks/controls transpiration
▪ Stomata may be present at irregular intervals
▪ A large number of multicellular hairs occur on the epidermis

o Cortex

▪ Cortex lies below the epidermis
▪ The cortex is differentiated into three zones
▪ The first zone (Hypodermis)
• Lies below the epidermis, consists of a few layers of

collenchyma cells

▪ The second zone
• Lies inner to the hypodermis
• Consists of a few layers of chlorenchyma cells
• Some resin ducts also occur in this zone
▪ The third zone
• Consists of parenchyma cells
• The innermost layer of the cortex is the endodermis
• Made of barrel shaped cells arranged compactly without

intercellular spaces

• Rich in starch grains, hence “starch sheath”
• In most dicot stems no casparian strips

[pic]

o The Stele

▪ In the central part of the stem inner to the endodermis
▪ It consists of pericycle, vascular bundles and pith
▪ Vascular bundles are arranged in a ring around the pith
• Pericycle

o Layers of cells between endodermis and vascular bundles

• Vascular Bundles

o Consist of xylem, phloem and cambium

o Xylem and phloem in stem occur together to form the vascular

bundles

o These vascular bundles are wedge shaped

o The vascular bundle is conjoint, collateral, open and endarch

o Phloem

• Consists of sieve tubes, companion cells and phloem

parenchyma

• Phloem conducts organic food materials from the leaves to

other parts of the plant body

o Cambium

• Consists of brick shaped and thin walled meristematic

cells

• Form new cells during secondary growth

o Xylem

• Consists of xylem fibres, xylem parenchyma, vessels and

tracheids

• Vessels are thick walled and arranged in a few rows
• Conducts water and minerals from the root to the other

parts of the plant body

• Pith (medulla)

o It is the large central portion of the stem

o It is composed of parenchyma cells with intercellular spaces

o The pith extends between the vascular bundles and form primary

medullary rays

o These extensions of the pith between the vascular bundles are

called

o It stores food

STEP 4: Internal Structures of the Monocot Stem (30 minutes)

• Internal structure of monocotyledonous stem consists of:

o Epidermis,

o Hypodermis,

o Ground tissue and

o Vascular bundles

▪ Epidermis
• It is the outermost layer of the stem made up of single layer of

parenchymatous cells and covered with a thick cuticle in some

plants

• Consists of few stomata
▪ Hypodermis
• Is made of few layer of sclerenchymatous cells lying below the

epidermis

• This layer gives mechanical strength to the plant
▪ Ground Tissue
• Consists of cells inner to the hypodermis
• Is not distinguished into zones like the dicot stem
• The cells contain reserve food material e.g. starch
• Contains the vascular bundles
• Carry out gaseous exchange function
▪ Vascular Bundles
• Vascular bundles are scattered in the ground tissue
• The vascular bundles are conjoint, collateral, endarch and

closed

• Vascular bundles are skull shaped
• Forms the woody part of the plant

STEP 5: Differences between Dicot and Monocot Stems (20 minutes)

|Activity: Small Group Discussion (10 minutes) |

| |

|DIVIDE students into small manageable groups |

| |

|ASK students to discuss on the following question |

| |

|What are the differences between monocot and dicot stems? |

| |

|ALLOW students to discuss for 10 minutes |

| |

|ALLOW few groups to present and the rest to add points not |

|mentioned |

| |

|CLARIFY and SUMMARIZE by using the contents below |

[pic]

STEP 6: Key Points (10 minutes)

• Stem is the part of both the flowering and nonflowering plants that is

usually above the ground, and in some plants below the ground. The stem

supports the leaves

• External features of the stem include presence of nodes, internodes,

lenticels, buds, leaf scars and bud scales

• Internally the stem is made up of layers of cells differentiated into

various function of support, storage, transport and photosynthetic in

some plants

STEP 7: Evaluation (5 minutes)

• What are the functions of the stem?
• What are the external structures of the stem?

References

Trease, G. E., Evans, W. C., & Evans, D. (2009). Trease and Evans

pharmacognosy. London: Saunders.

Joanne Barnes et al (2002), Herbal medicines 3rd Edition: Pharmaceutical

Press

Wallis, T. E. (2005). Textbook of pharmacognosy. New Delhi: CBS.

Robbers, J. E., Speedie, M. K., Tyler, V. E., & Tyler, V. E. (1996).

Pharmacognosy and pharmacobiotechnology. Baltimore: Williams &

Wilkins.

Heinrich, M., Kinghorn, A. D., Maizels, D., Gibbons, S., & Phillipson, J.

D. (2012). Fundamentals of pharmacognosy and phytotherapy. Edinburgh:

Churchill Livingstone/Elsevier.

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