Introduction to Microbiology – PST05103 Pharmaceutical Microbiology

NTA Level 5 • Semester 1 • PST05103

Introduction to Microbiology

Pharmaceutical Microbiology • Source Session/Topic 1
Full source-text version: all educational wording from the extracted learning source is retained; only presenter/tutor metadata and web-layout noise are removed, while formatting is improved for readability.

Session 1: Introduction to Microbiology

Total Session Time: 60 minutes

Pre-requisites

• Human anatomy and physiology

Students Learning Tasks

By the end of this session students are expected to be able to:

• Define terms used in microbiology
• Explain the history and development of microbiology

Resources Needed:

• Flip charts, marker pens, and masking tape
• Black/white board and chalk/whiteboard markers

SESSION OVERVIEW

|Step |Time |Activity/ |Content |

| | |Method | |

|1 |5 minutes |Presentation |Introduction, Learning Tasks |

|2 | |Presentation |Terminologies used in |

| |10 minutes | |Pharmaceutical Microbiology |

|3 |35 minutes |Presentation |History and development of |

| | | |microbiology |

|4 |5 minutes |Presentation |Key Points |

|5 |5 minutes |Presentation |Evaluation |

SESSION CONTENTS

STEP 1: Presentation of Session Title and Learning Tasks (5 minutes)

READ or ASK students to read the learning tasks and clarify

ASK students if they have any questions before continuing

STEP 2: Common Terminologies Used in Pharmaceutical Microbiology (10

minutes)

• Microorganisms

o Microorganisms are organisms that are too small to be seen by naked

eyes

o Microorganisms include viruses, bacteria, fungi, protozoa and algae.

• Microbiology
• Microbiology is the study of microorganisms which must be viewed

with the aid of a microscope or electron microscope

• Pharmaceutical microbiology

o This deals with the study of microorganisms of pharmaceutical

importance

• Bacteriology

o Is the study of bacteria

• Mycology

o Is the study of fungi

• Protozoology

o Is the study of protozoans

• Virology

o Is the study of viruses

STEP 3: History and Development of Microbiology (35 minutes)

• 1660’s Robert Hooke observed microorganisms for the first time with a

microscope and coined the term “cell”.

• 1632-1723 Anton van Leeuwenhoek credited with having observed the first

bacteria.

• 1828-1898 Ferdinand Cohn developed the first classification scheme based

on bacteria shape. Cohn detailed and described the life cycle of

Bacillus.

Cohn’s Classification System:

o Sphaerobacteria are spherically shaped.

o Microbacteria are rod shaped

o Desmobacteria are filamentous

o Spirobacteria are spiral shaped

• 1822-1895 Louis Pasteur Defined pasteurization to prevent spoilage of

food by bacteria, develop vaccines and disproved the scientific dogma of

“Spontaneous Generation”. He defined “Germ Theory” and demonstrated

that germs were responsible for disease.

• 1843-1910 Robert Koch identified anthrax and developed agar growth

medium. Koch’s postulates was a systematic method to establish the

microbial cause of disease.

• Ignaz Semmelweis was the first to recognize the need for good hygiene

during medical procedures. The first to identify nosocomial infections.

• 1827-1912 Joseph Lister developed antiseptic methods for use in surgery

and medicine.

• 1854-1915 Paul Ehrlich developed chemotherapy to cure infectious

diseases and discovers antibiotics to treat sleeping sickness and

syphilis.

• 1881-1951 Alexander Fleming discovered penicillin and lysozyme.
• 1864-1920 Dmitri Ivansvski discovered the first virus which is known as

the tobacco mosaic virus (TMV).

• 1952 Hershey & Chase Experiments identified that DNA was the genetic

material of bacteriophages.

• Hershey Case Experiment: using phage radioactively labeled with P32

(DNA) or S35 (protein) they infected bacteria cells. They found the P32

inside the bacteria not S35.

STEP 4: Key Points (5 minutes)

• Microorganisms are minute living things that are too small to be seen

by the naked eye.

• Microbiology is the scientific study of microorganisms
• Pharmaceutical microbiology is the scientific study of microorganisms of

pharmaceutical importance

STEP5: Evaluation (5 minutes)

• What is a microorganism?
• What is the difference between microbiology and biology?
• Who is the father of microbiology?
• Give important land marks in the history of microbiology

References

Hugo and Russell (2011), Pharmaceutical Microbiology 8th Edition, Willey-

Blackwel publications

Karen C. Carroll et al (2013); Jawetz, Melnick and Adelberg’s Medical

Microbiology 26th Ed. McGraw Hill Co. Inc.

Greenwood et al (2012); Medical Microbiology, 18th edition Churchill

Livingstone

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