The Plant Cell – PST05210 Basic Pharmacognosy

NTA Level 5 • Semester 2 • PST05210

The Plant Cell

Basic Pharmacognosy • Source Session/Topic 2
Full source-text version: all educational wording from the extracted learning source is retained; only presenter/tutor metadata and web-layout noise are removed, while formatting is improved for readability.

Session 2: The Plant Cell

Total Session Time: 120 minutes

Prerequisites

• None

Learning Tasks

By the end of this session students are expected to be able to:

• Explain structure of a plant cell
• List organelles of the plant cell
• List functions of plant cell organelles
• Explain the plant cell wall

Resources Needed:

• Flip charts, marker pens, and masking tape
• Black/white board and chalk/whiteboard markers
• LCD and laptop

SESSION OVERVIEW

|Step |Time |Activity/ |Content |

| | |Method | |

|1 |05 minutes |Presentation |Introduction, Learning Tasks |

|2 |25 minutes |Buzzing/ |Introduction to Plant Cells |

| | |Presentation | |

|3 |60 minutes |Brainstorming/|Functions of Internal structures |

| | |Presentation |of a plant cell |

|4 |20 minutes |Presentation |The plant cell wall |

|5 |05 minutes |Presentation |Key Points |

|6 |05 minutes |Presentation |Evaluation |

SESSION CONTENTS

STEP 1: Presentation of Session Title and Learning Tasks (5 minutes)

READ or ASK students to read the learning objectives and clarify

ASK students if they have any questions before continuing.

STEP 2: Introduction to Plant Cells (25 minutes)

|Activity: Buzzing (5 minutes) |

| |

|Ask students to brainstorm on the following question: |

| |

|What is a plant cell? |

| |

|ALLOW few students to respond? |

| |

|WRITE their responses on the flip chart/ board |

| |

|CLARIFY and SUMMARISE by using the content below |

[pic]

• A plant cell is the structural unit of the plant body
• The plant cells consist of internal and external structures
• The internal structures (organelles) include;

o The Nucleus

o Nucleolus

o Centrosome

o Ribosomes

o Rough Endoplasmic Reticulum

o Smooth Endoplasmic Reticulum

o Golgi Complex (Golgi apparatus or Golgi body)

o Mitochondria

o Plastids

o Proplastids

o Etioplasts

o Chloroplasts

o Chromoplasts

o Leucoplasts

▪ Amyloplasts
▪ Elaioplasts
▪ Proteinoplasts
▪ Statoliths

o Nucleoids

o Vacuoles

• The external structures are mainly the cell wall

STEP 3: Internal Structures of a Plant Cell (60 minutes)

|Activity: Brainstorming (5 minutes) |

|Ask students to brainstorm on the following question: |

| |

|What are the functions of these organelles? |

| |

|ALLOW few students to respond? |

| |

|WRITE their responses on the flip chart/ board |

| |

|CLARIFY and SUMMARISE by using the content below |

• The Nucleus

o Spherical body containing a nucleolus

o Surrounded by the nuclear membrane

o Contains DNA (genetic material in chromosomes)

• Nucleolus

o An organelle within the nucleus where ribosomal RNA is produced

o It controls many of the functions of the cell (by controlling protein

synthesis)

• Centrosome

o It is a small body located near the nucleus that forms and

organizes microtubules

o Microtubules are important in cell division (mitosis)

• Ribosomes

o Are small organelles composed of RNA-rich cytoplasmic granules that are

sites of protein synthesis

• Endoplasmic Reticulum

o Endoplasmic reticulum is a system of interconnected, membranous,

infolded and convoluted sacks located in cytoplasm (the ER is

continuous with the outer nuclear membrane)

o It transports materials through the cell and produces proteins

o Smooth Endoplasmic Reticulum are smooth (i.e. no ribosomes on surface)

▪ Contains enzymes and produces and digests lipids (fats) and

membrane proteins

• Golgi Complex: (i.e. Golgi apparatus or Golgi body)

o Is a flattened, layered, sac-like organelle that looks like a stack of

pancakes, located near the nucleus

o It packages and transports materials (proteins and carbohydrates) to

different locations inside/outside cell

• Mitochondria

o Spherical to rod-shaped organelles with a double membrane that

generates energy for the cell

• Plastids

o Are structures responsible for photosynthesis, storage of products like

starch and for the synthesis of many classes of molecules such as fatty

acids etc. needed as cellular building blocks and/or for the function

of the plant. There several types; proplastids, etioplasts,

chloroplasts, chromoplasts, leucoplasts and leucoplasts specialized for

different functions in the plant body.

• Vacuoles

o Are regions bound by membrane and filled with cell sap and surrounded

by the tonoplast

o In mature living cells may compose 90 – 95% of cell volume

o Storage site for variety of (ergastic) substances such as:

▪ Water
▪ Enzymes and other proteins
▪ Water-soluble pigments (e.g. red and blue anthocyanins)
▪ Toxic alkaloids, tannins
▪ Lipids
▪ Crystals (calcium oxalate, calcium carbonate, silicon dioxide)
▪ Organic acids (oxalate, malate)

STEP 4: The Plant Cell Wall (20 minutes)

• The plant cell wall is heterogeneous structure composed of layers

(lamellae)

o Each cell secretes its own wall

o Junction between the walls is the Middle lamella

o Cell wall is penetrated by plasmodesmata connecting neighbouring cells

o Cell walls are primarily composed of sugar polymers i.e.

polysaccharides

o The architecture, mechanics, and function of plants depend crucially on

structure of cell wall

• Functions

o Regulates cell volume by limiting protoplast size preventing cell

rupture

o Determines cell shape

o Involved in cell-cell adhesion; provide support to cells and whole

plant

o Forms specialized structures for:

▪ Transport of water and nutrients
▪ Defense against bacterial and fungal attack
▪ Reproduction
• Composition of Cell Walls

o Primary cell wall:

▪ Which is the only cell wall in growing and dividing cells
▪ First formed cell wall
▪ Composed of cellulose embedded in matrix of hemicellulose and

pectin, cellulose molecules: Unbranched glucose chains and

hemicellulose – a highly branched molecule of sugars and sugar

derivatives

▪ Pectins:
• Are highly branched negatively charged polysaccharides rich in

galacturonic acid

• Give compressive strength to the cell wall
• Modulate porosity, pH, and ion concentrations within the wall

o Primary wall/Middle Lamellae

▪ Primarily associated with hemicelluloses and pectins
▪ Water is 65% of weight of cell wall

o Secondary Cell Wall

▪ Many plant cells synthesize secondary walls after the cell has

completely elongated

▪ Secondary wall is deposited by protoplast inside primary wall after

cell stops growing

▪ Composition of secondary wall:
• Cellulose, hemicellulose and pectin
• Lignin
• Stronger than cellulose
• There substances found on the surface of the cell wall. These include

cuticle waxes, suberin and minerals

STEP 5: Key Points (5 minutes)

• The main reason of studying the pant cell is to understand parts involved

in synthesis and storage of drugs

• The plant cell is the structural unit of the plant
• The plant cell is composed of internal and external structures
• The cell wall is a rigid external layer of the plant cell that protects

the cell

STEP 6: Evaluation (5 minutes)

• Mention organelles found in a plant cell
• What is the composition of the primary cell wall?
• What are the functions of the Golgi apparatus?

References

Trease, G. E., Evans, W. C., & Evans, D. (2009). Trease and Evans

pharmacognosy. London: Saunders.

Joanne Barnes et al (2002), Herbal medicines 3rd Edition: Pharmaceutical

Press

Wallis, T. E. (2005). Textbook of pharmacognosy. New Delhi: CBS.

Robbers, J. E., Speedie, M. K., Tyler, V. E., & Tyler, V. E. (1996).

Pharmacognosy and pharmacobiotechnology. Baltimore: Williams &

Wilkins.

Heinrich, M., Kinghorn, A. D., Maizels, D., Gibbons, S., & Phillipson, J.

D. (2012). Fundamentals of pharmacognosy and phytotherapy. Edinburgh:

Churchill Livingstone/Elsevier.

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